Plant Proteomics: Methods and Protocols

This detailed new edition reflects the increasing diversity of biological materials, analytical strategies, and computational tools currently employed in plant proteomic research. Spanning the entire proteomics workflow, this book explores subjects from sample preparation and protein extraction to advanced mass spectrometry analyses, data validation, functional interpretation, and integration with complementary omics disciplines, with special attention given to non-model and orphan plant species, which continue to present unique analytical and bioinformatic challenges but also offer valuable opportunities for translational research in agriculture, food science, and environmental studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Proteomics: Methods and Protocols, Fourth Edition serves as an ideal guide for graduate students entering the field as well as experienced scientists seeking to implement new technologies or refine existing workflows.