Botrytis cinerea: Methods and Protocols

This volume provides comprehensive protocols on Botrytis cinerea and the main methods used to study this important fungal species. Chapters detail cover classical approaches, including isolation, cultural techniques, sclerotia induction, storage, microscopy and protein localization, inoculation assays on fruits and vegetables, antifungal susceptibility testing, protoplast production and transfection, RNA interference, and identification of mycoviral infections. Additionally, the volume presents state-of-the-art molecular and omics-based approaches, including high-quality DNA and RNA extraction, molecular marker development, genome assembly, quantitative gene expression analysis, and integrative transcriptomic, proteomic, and metabolomic analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Botrytis cinerea: Methods and Protocols aims to promote experimental rigor, facilitate cross-disciplinary collaboration, and stimulate innovative research directions in Botrytis biology.