Molecular Markers in Plants: Methods and Protocols

This detailed volume provides a comprehensive yet accessible account of various molecular marker systems and their practical applications. Accounting for early methods like Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD), the book explores Single Nucleotide Polymorphism (SNP) markers and high-throughput sequencing technologies that have further revolutionized the field, enabling large-scale genomic analysis with remarkable accuracy. Each method is described with careful attention to essential steps such as DNA extraction, quantification, amplification, and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Markers in Plants: Methods and Protocols serves as an ideal resource to inspire further exploration and innovation in the use of molecular markers, leading to the effective application of these techniques to sustainable agriculture and biodiversity conservation.