Aspergillus fumigatus: Methods and Protocols

This book presents current molecular biology methods involving Aspergillus fumigatus for researchers in mycology, fungal genetics, and medical microbiology looking to deepen their understanding of this species of fungus at the molecular level. Sections of the book explore approaches to CRISPR-based genome editing, methods for identification, genotyping, and detection, assays for gene regulation, protocols for electrophoretic mobility shift assays and chromatin immunoprecipitation, as well as models to study host-pathogen interactions and methods to investigate the fungal cell wall and secreted components. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Aspergillus fumigatus: Methods and Protocols serves as an ideal guide for scientists attempting to shorten the time spent on routine problems at the bench and move more quickly from idea to results.